Gene gun application in the generation of effector T cells for adoptive immunotherapy
نویسندگان
چکیده
منابع مشابه
Divergent roles for CD4+ T cells in the priming and effector/memory phases of adoptive immunotherapy.
The requirement for CD4(+) Th cells in the cross-priming of antitumor CTL is well accepted in tumor immunology. Here we report that the requirement for T cell help can be replaced by local production of GM-CSF at the vaccine site. Experiments using mice in which CD4(+) T cells were eliminated, either by Ab depletion or by gene knockout of the MHC class II beta-chain (MHC II KO), revealed that p...
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Effective T-cell therapy against cancer is dependent on the formation of long-lived, stem cell-like T cells with the ability to self-renew and differentiate into potent effector cells. Here, we investigated the in vivo existence of stem cell-like antigen-specific T cells in allogeneic stem cell transplantation (allo-SCT) patients and their ex vivo generation for additive treatment posttransplan...
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BACKGROUND Insufficient persistence and effector function of chimeric antigen receptor (CAR)-redirected T cells have been challenging issues for adoptive T cell therapy. Generating potent CAR T cells is of increasing importance in the field. Studies have demonstrated the importance of the Akt pathway in the regulation of T cell differentiation and memory formation. We now investigate whether in...
متن کاملGENE THERAPY Human effector CD8 T cells derived from naive rather than memory subsets possess superior traits for adoptive immunotherapy
Cluster of differentiation (CD)8 T cells exist as naive, central memory, and effector memory subsets, and any of these populations can be genetically engineered into tumor-reactive effector cells for adoptive immunotherapy. However, the optimal subset from which to derive effector CD8 T cells for patient treatments is controversial and understudied. We investigated human CD8 T cells and found t...
متن کاملHost lymphodepletion augments T cell adoptive immunotherapy through enhanced intratumoral proliferation of effector cells.
T-cell adoptive immunotherapy for stringent murine tumor models, such as intracranial, s.c., or advanced pulmonary metastases, routinely uses lymphodepletive conditioning regimens before T-cell transfer, like recent clinical protocols. In this study, we examined whether host lymphodepletion is an obligatory component of curative T-cell therapy; we also examined the mechanism by which it augment...
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ژورنال
عنوان ژورنال: Cancer Immunology, Immunotherapy
سال: 2000
ISSN: 0340-7004,1432-0851
DOI: 10.1007/s002620050012